TABLE 2.
Oligonucleotides used in this study
| Purpose and oligonucleotide | Sequence | Reference |
|---|---|---|
| Cloning | ||
| D4F | 5′ CGGTGAACATATGAATTCCAGCAA 3′ | |
| D4-SD-F | 5′ CTCATCATACATATGCGCAATC 3′ | |
| D4R | 5′ TCACTCGAGGCATCAGCCTG 3′ | |
| AAD_Nde | 5′ AATACATATGGAGCGGAAGACTCATTGT 3′ | |
| D4AAD_For_Bam | 5′ AATAGGATCCGGAGCGGAAGACTCATTGT 3′ | |
| D4AAD_For_Sac | 5′ AATAGAGCTCGGAGCGGAAGACTCATTGT 3′ | |
| D4AAD_Rev | 5′ AAACTCGAGTCATAGAGCTCCCCTCCGGAGATCGTAAAC 3′ | |
| D4AAD_Rev_Sac | 5′ AATAGAGCTCCGCCTCCGGAGATCGTAAAC 3′ | |
| ΔAAD_Rev_Sac | 5′ TGAAGAGCTCTCAGAATCTAGG 3′ | |
| ΔAAD_For | 5′ AATAGAGCTCCAAGAAGACCTGCATTTAT 3′ | |
| CAAD-5 | 5′ GGAATTCCATATGTTGTCAAATAGTGATACG 3′ | |
| CAAD-3 | 5′ CCGCTCGAGTTAATTCCAGGAGTCAATATC 3′ | |
| CAAD-D1 | 5′ CGGAGCTCCGCGTGTTTAAGTCTAAGACCTT 3′ | |
| CAAD-D2 | 5′ GCGAGCTCCGAAGAAAAAACAGCAGTG 3′ | |
| pcfC_F_5 | 5′ AAACTGTCATATGCAGACGTAC 3′ | |
| pcfCΔN103-F | 5′ GCTCGTTTTCATATGGCTACACCTGC 3′ | |
| pcfC-R-XhoI | 5′ AACGCACGTCCTCGAGCTATTAGGCTAGAACGTGTGTTTC 3′ | |
| ΔN103R | 5′ ACATGCATGCCTGCAGCTCGAGTTAAACGTGTGTTTCTTCTT 3′ | |
| GST-pcfC-Δ103-F | 5′ CATGGTCTCGGATCCGCTCGTTTTGCTACACCTGC 3′ | |
| CAAD_For_Sac | 5′ CGGAGCTCGTTGTCAAATAGTGATACG 3′ | |
| CAAD_Rev_Sac | 5′ GCGAGCTCGAATTCCAGGAGTCAATATC 3′ | |
| D4AAD_For_Sac | 5′ AATAGAGCTCGGAGCGGAAGACTCATTGT 3′ | |
| D4AAD_Rev_Sac | 5′ AAACTCGAGTCATAGAGCTCCCCTCCGGAGATCGTAAAC 3′ | |
| EMSAs | ||
| pCF10-S | 5′ AAATTCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCG3′ | 30 |
| pCF10-AS | 5′GATCCGGCTTTCTTTTTGCAAGCGGAGCAACATGCTAGCATGTTGCG 3′ | 30 |
| RB-S | 5′ AGCTCAAATTACAACGGTATATATCCTGCCAGTCAGCATCATCACACCAA 3′ | |
| RB-AS | 5′TTGGTGTGATGATGCTGACTGGCAGGATATATACCGTTGTAATTTGAGCT 3′ |