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. 2015 Jun 19;197(14):2335–2349. doi: 10.1128/JB.00189-15

TABLE 2.

Oligonucleotides used in this study

Purpose and oligonucleotide Sequence Reference
Cloning
    D4F 5′ CGGTGAACATATGAATTCCAGCAA 3′
    D4-SD-F 5′ CTCATCATACATATGCGCAATC 3′
    D4R 5′ TCACTCGAGGCATCAGCCTG 3′
    AAD_Nde 5′ AATACATATGGAGCGGAAGACTCATTGT 3′
    D4AAD_For_Bam 5′ AATAGGATCCGGAGCGGAAGACTCATTGT 3′
    D4AAD_For_Sac 5′ AATAGAGCTCGGAGCGGAAGACTCATTGT 3′
    D4AAD_Rev 5′ AAACTCGAGTCATAGAGCTCCCCTCCGGAGATCGTAAAC 3′
    D4AAD_Rev_Sac 5′ AATAGAGCTCCGCCTCCGGAGATCGTAAAC 3′
    ΔAAD_Rev_Sac 5′ TGAAGAGCTCTCAGAATCTAGG 3′
    ΔAAD_For 5′ AATAGAGCTCCAAGAAGACCTGCATTTAT 3′
    CAAD-5 5′ GGAATTCCATATGTTGTCAAATAGTGATACG 3′
    CAAD-3 5′ CCGCTCGAGTTAATTCCAGGAGTCAATATC 3′
    CAAD-D1 5′ CGGAGCTCCGCGTGTTTAAGTCTAAGACCTT 3′
    CAAD-D2 5′ GCGAGCTCCGAAGAAAAAACAGCAGTG 3′
    pcfC_F_5 5′ AAACTGTCATATGCAGACGTAC 3′
    pcfCΔN103-F 5′ GCTCGTTTTCATATGGCTACACCTGC 3′
    pcfC-R-XhoI 5′ AACGCACGTCCTCGAGCTATTAGGCTAGAACGTGTGTTTC 3′
    ΔN103R 5′ ACATGCATGCCTGCAGCTCGAGTTAAACGTGTGTTTCTTCTT 3′
    GST-pcfC-Δ103-F 5′ CATGGTCTCGGATCCGCTCGTTTTGCTACACCTGC 3′
    CAAD_For_Sac 5′ CGGAGCTCGTTGTCAAATAGTGATACG 3′
    CAAD_Rev_Sac 5′ GCGAGCTCGAATTCCAGGAGTCAATATC 3′
    D4AAD_For_Sac 5′ AATAGAGCTCGGAGCGGAAGACTCATTGT 3′
    D4AAD_Rev_Sac 5′ AAACTCGAGTCATAGAGCTCCCCTCCGGAGATCGTAAAC 3′
EMSAs
    pCF10-S 5′ AAATTCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCG3′ 30
    pCF10-AS 5′GATCCGGCTTTCTTTTTGCAAGCGGAGCAACATGCTAGCATGTTGCG 3′ 30
    RB-S 5′ AGCTCAAATTACAACGGTATATATCCTGCCAGTCAGCATCATCACACCAA 3′
    RB-AS 5′TTGGTGTGATGATGCTGACTGGCAGGATATATACCGTTGTAATTTGAGCT 3′