FIG 1.

An E. coli ΔyqjA mutant fails to grow at elevated pH. (A) Parent E. coli W3110 and a ΔyqjA mutant were grown to mid-log phase at pH 7 and inoculated into liquid LB medium at pH 7.0, 8.0, 8.25, 8.5, 8.75, 9.0, 9.25, 9.5, or 9.75. Growth at 37°C was monitored hourly. (B) Parent strain W3110 and a ΔyqjA mutant were grown to mid-log phase at pH 7.0, and then 5 μl of serially diluted cells was spotted onto LB medium plates at pH 7.0, 8.5, 8.75, 9.0, 9.25, and 9.5, and the plates were incubated at 37°C for 20 to 24 h. The error bars indicate standard deviations of three independent measurements.