TABLE 2.
Oligodeoxynucleotide primers used in this work
| Primer | Sequencea (5′–3′) |
|---|---|
| orf398-7120-5 | CCAAAGCCATGGCGAATAATATCGGTC |
| narB-7120-3 | CTACCAAAACCCTATGTCC |
| narB-7120-4 | GCGAATAGTGGCGAATTACC |
| narB-7120-5 | GCTCGAGCGTCGAGGTAGCGCCAAG |
| narB-7120-6 | CCACTGAGATGACTGGAG |
| narB-7120-8 | CGACGTTCTAGAATCTACCAAAACC |
| narB-7120-11 | AACAGGTACCCACTGAGATGACTGGAG |
| alr0613-1 | GGCAAATTTTGGGAATCACG |
| alr0613-2 | GACAGAGCTAAAATTGCCAC |
| alr0613-4 | CGTGATTCCCAAAATTTGCC |
| alr0613-5 | CACGGATCCGGGCGTGGCAATTTTAGCTC |
| alr0613-6 | GCCACGCCCGGATCCGTGATTCCCAAAATTTGCC |
| alr0614-1 | CAGATTTTGTTGATTCCCTGCG |
| alr0614-2 | AAACTCGGCGATCGCCTTGGG |
| alr0614-3 | ACTCGAGGATTGAGTTAGTAGTAAGGAC |
| alr0614-4 | GAATTCTGCCATAAATGAGCG |
| alr0614-9 | ATTCTGCAGTGAGTTCTTTGAATTTGAATCAG |
| alr0614-10 | ATTGAATTCCCTATTCTACTTATATTAAACTCG |
| pKT25-3 | GTCATGACTCTAGAGGATCCCGGGGCATGCCTAAGTAACTAAG |
| pKT25-4 | AATTCTTAGTTACTTAGGCATGCCCCGGGATCCTCTAGAGTCATGACTGCA |
| pUT18-3 | GGTCGACCATGGAGGATCCCCGGGTACCGAGCTCG |
| pUT18-4 | AATTCGAGCTCGGTACCCGGGGATCCTCCATGGTCGACCTGCA |
a
Introduced restriction enzyme-cutting sites are shown in boldface.