FIG 5.
HSV-1 genome copy number is reduced by 10-fold in LULL1 knockout cells. DNA samples from HSV-1-infected parental, LULL1 knockout, and LULL1 rescue HeLa cells were analyzed by quantitative PCR (qPCR) using primers against both the viral gene ICP27 and the cellular housekeeping gene GAPDH. ΔCp values were calculated by subtracting the GAPDH Cp values from the corresponding ICP27 ΔCp values in order to obtain a ΔCp value that directly reflects the number of viral genomes per cell. The resulting ΔCp values were normalized to the 2-hpi time point, and these ΔΔCp values were plotted for each of the three cell lines at both 18 and 24 hpi.