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PCR analysis of ASFV-G-Δ9GL virus DNA using specific primers targeting the 9GL (B119L), p72 (B646L), or β-GUS genes. (A) Assessment of purity of the ASFV-G-Δ9GL virus stock by PCR. Lane 1, ASFV-G-Δ9GL; lane 2, ASFV-G. (B) Identification of the presence of parental ASFV-G (lanes 1 to 4) in viruses isolated from animals infected with 104 HAD50 ASFV-G-Δ9GL virus. The control (lane C) consists of a plasmid containing the respective target genes.
