FIG 5.

Evaluating the efficacy of the different injection routes for delivering Ad-porcine IFN-αγ and Ad-3siRNA in combination in swine. Pigs were inoculated by aerosol, i.m. injection, or aerosol and i.m. injection combined with 3 × 10⁹ TCID₅₀ each of Ad-porcine IFN-αγ and Ad-3siRNA. At 48 h postinjection with the recombinant adenoviruses, pigs were challenged with O/Andong/SKR/2010 by direct contact with donors. The maximum amount of FMDV detected in serum (A) and saliva swab (B) specimens was measured by real-time RT-PCR from the day that FMDV was first detected in serum (C) or saliva swab (D) specimens to 7 DPC. The symbols (▼, ▽, ●, ○, and ■) represent duplicate values of viral copy numbers. (E) Clinical signs at 7 DPC are indicated as clinical scores. Mean values are indicated with bars. A t test was performed to identify statistically significant differences. *, P < 0.05; **, P < 0.005.