FIG 6.

AcMNPV infection or p35 expression does not block degradation of dsRNA. (A) Northern blot analysis of dsGFP levels at times post-AcMNPV infection, using a probe specific to GFP. rRNA is shown as a loading control. (B) Northern blot analysis of dsGFP in Sf9 cells transfected with the pIZ empty vector and pIZ/p35. (C) Northern blot analysis of small RNAs from Sf9 cells cotransfected with dsGFP and the pIZ empty vector or pIZ/p35. The blot was rescanned only in the small RNA area (boxed) to enhance detection of siRNAs by phosphorimager analysis (shown by arrow). tRNAs are shown as a loading control. MW, molecular weight. (D) Western blot analysis of Sf9 cells transfected with pIZ/GFP only or transfected with pIZ/GFP and GFP siRNAs (siGFP) plus mock, the pIZ empty vector, or pIZ/p35. The blot was probed with an anti-GFP antibody and anti-Hsp70 to show equal loading of the samples.