FIG 8.

Infectivity of HIV-1 WT and L92P virions. (A) Equal virion amounts of the WT HIV-1 and L92P viruses, as determined by RT-qPCR, were used to infect MAGI cells. At 2 days postinfection, the MAGI protocol was performed (see Materials and Methods), and three wells from each infection were inspected under light microscopy for positive blue cells. Scale bar, 50 μm. (B) The results of three independent MAGI experiments were quantified, showing the means ± the SD. Mock, infection with supernatants obtained from uninfected HEK293 cells. (C) JLTRG-R5 cells were infected with equal virion amounts of WT HIV-1 or the L92P mutant. Once a day, the level of GFP, produced in the infected cells, was quantified in a plate reader, as described in Materials and Methods. The numbers represent the mean results of three independent experiments ± the SD.