FIG 5.

Neutralization susceptibility of SIVsm Envs to autologous sooty mangabey plasma. Six Envs cloned from experimentally SIV-infected sooty mangabeys (FJv, FFs) or naturally SIV-infected sooty mangabeys (FFv, FWk, FBn, FNg) were assessed for neutralization by a sample of autologous plasma collected 0.5 to 4 years later using the Tzm-bl cell assay (39). The percentage of residual infectivity against 1:20-diluted plasma is shown on the vertical axis. Residual infectivity of 100% represents no inhibition, while 0% residual infectivity represents complete inhibition. Each bar is color coded according to the neutralization tier of the Env pseudoviruses. Each Env pseudovirus-plasma combination was tested in duplicate wells in at least two independent experiments. The standard error of the mean is shown for each Env pseudovirus.