FIG 3.

The N37 of E2 is critical for its interaction with Trx2. (A) Sequence alignment of the E2 protein between the CSFV Shimen strain and C-strain. (B) Schematic representation of the chimeric E2 mutants between Shimen strain and C-strain expressed in HEK293T cells. (C) GST pulldown analysis of the interaction of GST-Trx2 with the Flag-tagged chimeric E2 mutants expressed in HEK293T cells. The glutathione-Sepharose 4B resin (catalog no. 10049253; GE Healthcare) conjugated with GST or GST-Trx2 was incubated with the recombinant Flag-tagged SME2, CE2, or chimeric E2 expressed in HEK293T cells. After washing with PBS, the bound proteins were subjected to SDS-PAGE and immunoblotting analysis. (D) Sequence alignment of the Trx2-binding sites in the E2 proteins of different CSFV strains.