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. 2015 Jun 3;89(16):8428–8443. doi: 10.1128/JVI.00997-15

FIG 10.

FIG 10

Effects of tetrandrine. (A to E, G, and H) 293A cells (or NIH 3T3 cells for ecotropic MLV) were incubated with pseudotyped lentiviral or retroviral vectors expressing fusion proteins from arenavirus NW clade B (A); NW recombinant clade A/B (B); NW clade A (C); NW clade C (D); OW arenaviruses (E); JUNV, Cd1, or chimeras (G); and fusion proteins from other virus families (H). Cells were treated with DMSO or tetrandrine, and relative titers are shown as percentages of the control titer (DMSO treated). Mean titers (in TU per milliliter) on DMSO-treated cells were as follows: 2.7 × 105 for JUNV, 5.0 × 104 for MACV, 1.1 × 105 for TCRV, 5.5 × 104 for GTOV, 5.6 × 104 for AMAV, 8.1 × 103 for WWAV, 8.2 × 103 for BBTV, 8.8 × 103 for SKTV, 7.5 × 103 for FLEV, 3.4 × 105 for PICV, 3.9 × 104 for OLVV, 7.5 × 103 for LATV, 2.1 × 106 for LASV, 4.0 × 105 for LCMV13, 3.3 × 105 for LCMV53b, 8.4 × 104 for JUNV XJ, 7.9 × 103 for Cd1, 1.7 × 104 for C1X2, 8.2 × 103 for X1C2, 1 × 106 for VSV, 3.7 × 104 for amphotropic MLV, 8.2 × 104 for ecotropic MLV, 4.7 × 104 for Ebola virus, 1.9 × 103 for influenza virus, 2.8 × 104 for Hendra virus, and 3.4 × 105 for Nipah virus. All data are the means ± standard deviations of results from 2 to 4 independent experiments. (F) Quantitation of Cd1 and LCMV-4 infection (number of foci) of Vero E6 cells treated with DMSO alone, ribavirin, or tetrandrine. Mean titers on DMSO-treated cells were 1 × 104 FFU/ml and 2.3 × 104 FFU/ml for Cd1 and LCMV, respectively. All data are the means ± standard deviations of results from 2 to 7 independent experiments. Statistical significance compared to the DMSO control is indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).