FIG 3.

Effects of compounds 1 to 3 on arenavirus replication. (A and B) Focus-forming assays. Vero E6 monolayers were infected with Cd1 or LCMV-4, with the indicated compounds or the DMSO control being present before and after virus incubation. Cells were analyzed 48 h later by using specific antibodies and immunofluorescence to detect foci of infection. Images are representative of data from 2 to 5 experiments. (C and D) Quantitation of virus infections (FFU per milliliter) of cells treated with DMSO or compounds compared to the DMSO-treated control ± standard deviations from 2 to 5 independent experiments. Mean titers in DMSO-treated control cells were 1 × 10⁴ FFU/ml (Cd1) and 2.3 × 10⁴ FFU/ml (LCMV-4). All data from panel C were statistically significant compared to the DMSO control (P < 0.0001), and in panel D, statistical significance compared to the DMSO control is indicated (****, P < 0.0001).