FIG 7.
Effects of compounds 1 to 3 on MACV IM binding to cells. (A) Schematic representation of the IM binding assay. (B) A total of 5 × 105 K562 cells were incubated with MACV IM supernatants, or a control supernatant (Ctrl.) from cells transfected with an Fc-only construct, in the presence of DMSO or compounds, as indicated. IM binding was determined by flow cytometry for Alexa Fluor 488 and is shown relative to that of DMSO-treated cells (100%). The mean percentage of Alexa Fluor 488-positive cells on DMSO-treated IM cells was 93.7%. All data represent the means ± standard deviations from 2 to 3 independent experiments.