FIG 1.
Recombinant virus design. (A) Amino acid alignment used to generate the consensus sequences. Numbering is based on the consensus. Amino acid disagreements from the consensus are indicated. The final QKL residues were not included in the consensus used for cloning. The duplication spans residues 258 to 277. (B) Subclones harboring the designed consensus sequences of BA G or BA GΔdup were obtained from GeneArt. SacI and SacII restriction sites flanking the G genes were utilized to clone the G constructs into pSynkRSVline19F in place of A2 G. Important domains of G are labeled, and the amino acid sequence of the duplication is shown, with the initial run of amino acids in regular type and the inexact duplication in italics.