FIG 5.
Competitive-infection assay. HEp-2 cells were infected with A2-K-BAG-line19F, A2-K-BAGΔdup-line19F, or both viruses at a ratio of 1:1, 10:1, or 1:10. Twenty-four and 48 h postinfection, RNA was harvested from cells and subjected to reverse transcription (RT)-PCR to amplify a portion of the G gene. Equal amounts of RNA were used for the reverse transcription, and equal volumes of cDNA were used as templates for the PCR. Gels are representative of two experiments. Expected PCR product sizes are 709 bp for A2-K-BAG-line19F and 649 bp for A2-K-BAGΔdup-line19F.