TABLE 2.
Average number of raw sequences per consensus Primer ID as input template varies for a fixed number of raw readsa
| No. of RNA templatesb | No. of template consensus reads | Avg no. of raw sequences per consensus Primer ID (SD)c |
|||
|---|---|---|---|---|---|
| Observed | Model 1 | Model 2 | Model 3 | ||
| 370 | 102 | 2,756 (2,114) | 2,703 (53) | 2,497 (45) | 2,712 (1,631) |
| 1,111 | 423 | 662 (418) | 901 (30) | 832 (29) | 834 (470) |
| 3,333 | 1,126 | 247 (124) | 302 (29) | 278 (25) | 284 (161) |
| 10,000 | 2,911 | 90 (41) | 102 (18) | 94 (16) | 96 (55) |
a
This analysis used 300,000 quality raw sequences.
b
A 30% conversion rate of RNA templates to cDNA templates was applied in the simulation models.
c
Observed, observed data from the serial dilution experiment; model 1, no PCR bias, no error in the Primer ID region; model 2, no PCR bias, error rate in the Primer ID region = 0.01; model 3, PCR bias (first 10 cycles of PCR, only 50% of templates were used), error rate in the Primer ID region = 0.01.