TABLE 4.
Poisson distribution of expected substitutions and observed substitutions per position from sequencing the 8E5 viral RNA control samplesa
| No. of substitutions per position | RSB14 R1 V1/V2 region (265 bp)b |
RSB14 R2 C2/V3 region (253 bp)c |
||
|---|---|---|---|---|
| No. of positions from the Poisson distribution | No. of positions observed | No. of positions from the Poisson distribution | No. of positions observed | |
| 0 | 166 | 169 | 89 | 95 |
| 1 | 78 | 75 | 93 | 89 |
| 2 | 18 | 18 | 49 | 42 |
| 3 | 3 | 1 | 17 | 20 |
| 4 | 0 | 1 | 4 | 5 |
| 5 | 0 | 0 | 1 | 1 |
| 6 | 0 | 1 | 0 | 0 |
| 7 | 0 | 0 | 0 | 1 |
a
The total number of consensus sequences was 13,471. The first and last two bases of the C2/V3 region were excluded from this analysis.
b
The total observed errors were 124 for the V1/V2 region. The Poisson distribution was calculated based on the observed error rate, which for this experiment was measured at 0.003%.
c
The total observed errors were 265 for the C2/V3 region. The Poisson distribution was calculated based on the observed error rate, which for this experiment was measured at 0.008%.