FIG 1.

OSMI-1 inhibits OGT activity in HFFs. (A) Chemical structure of OSMI-1. (B) Effect of OSMI-1 on O-GlcNAcylation in HFF cells. HFFs were incubated with increasing concentrations of OSMI-1 for 24 h. Cell lysates were analyzed by immunoblotting using O-GlcNAc-specific RL2 antibody. (C) HFFs were treated with increasing concentrations of OSMI-1. After 24 h, cell viability was measured with CellTiter-Glo Luminescent Cell Viability assay (Promega) and expressed as a percentage of DMSO-treated control cells.