FIG 3.

Effect of OSMI-1 on HSV-2 replication. (A) HFFs were infected with WT HSV-2 at MOIs of 0.1 and 5 and treated with 50 μM OSMI-1 or DMSO. Viral yields were determined at 24 hpi (MOI of 5) and 48 hpi (MOI of 0.1) by plaque assays on Vero cells. Means ± SEMs (n = 3) were analyzed by one-way ANOVA with repeated measures followed by Bonferroni posttest. *, P < 0.05. (B) HFFs were infected with WT HSV-2 or 186 TK⁻ virus at an MOI of 0.1, followed by addition of 50 μM OSMI-1, 100 μM acyclovir (ACV), or DMSO control. Virus yield was determined at 24 hpi by titration on Vero cells. Bars represent mean values ± SEMs from three independent experiments.