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. 2015 May 27;89(16):8162–8181. doi: 10.1128/JVI.00469-15

FIG 7.

FIG 7

Effect of siRNA targeting the Wave2 multicomplex on Gag VLP production. Jurkat T cells were transfected with pGag and with the siRNA control or siRNA against Rac1, Pak2, Wave2, IRSp53, or Arp3. (A) Immunoblot analysis of pr55Gag in cell lysates and in viral particles. Tubulin was using as a loading control. (B) Extracellular virus production measured by quantification of immunoblot images, corresponding to the ratio of extracellular pr55Gag to intracellular pr55Gag. Bars show mean values and standard deviations resulting from four independent experiments. The statistical significances of differences were calculated by an unpaired t test. **, P value of <0.01. (C) Cell viability evaluated by dead cell counting using trypan blue. (D) Quantification of protein depletion after siRNA treatment. Bars show mean values and standard deviations resulting from four independent experiments.