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. Author manuscript; available in PMC: 2015 Aug 4.
Published in final edited form as: Bioorg Med Chem. 2014 Jan 30;22(5):1620–1625. doi: 10.1016/j.bmc.2014.01.034

Figure 2.

Figure 2

Kinase-catalyzed labeling and subsequent photocrosslinking with ATP–ArN3.1 CK2 kinase was incubated with full length α-casein in the presence of ATP 1 or ATP–ArN3 2 before separation by SDS–PAGE and visualization with a CK2 antibody. Reactions were incubated either with or without exposure to 365 nm light (UV). Trifluoroacetic acid was added to a final concentration of 50% after crosslinking to cleave the phosphoramidate bond of the crosslink. The expected 68 kDa crosslinked complex is indicated as CK2-casein. The gel is representative of at least three independent trials.