Skip to main content
. Author manuscript; available in PMC: 2016 Sep 17.
Published in final edited form as: Toxicol Lett. 2015 Jul 3;237(3):228–236. doi: 10.1016/j.toxlet.2015.06.1708

Fig. 3. Effects of BaP treatment on transcriptional and epigenetic status of the ER gene proximal promoter region.

Fig. 3

(A) BaP treatment significantly decreased the binding of NR2E3 relative to the control. MCF-7 cells were treated with BaP (5 μM) for 90 min and determined by ChIP-PCR. (B) The mice were injected twice with BaP (125 mg/kg) and sacrificed 96 h later. The livers were homogenized, and the NR2E3 and ER protein levels were analyzed on immunoblots of cell lysates probed with anti-NR2E3 and anti-ERα antibodies. The livers were homogenized and lysed, and the total RNA was prepared for the PCR analysis of NR2E3 and ERα mRNA expression relative to the GAPDH expression. (C) BaP treatment significantly decreased the binding of NR2E3 relative to the control, as determined by ChIP-PCR. Results are means ± SE for at least 3 replicated determinations, and significantly (P < .05) increased/attenuated (*) or decreased (**) responses are indicated.