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. Author manuscript; available in PMC: 2016 Sep 17.
Published in final edited form as: Toxicol Lett. 2015 Jul 3;237(3):228–236. doi: 10.1016/j.toxlet.2015.06.1708

Fig. 4. Effects of NR2E3 loss on transcriptional and epigenetic status of the ER gene proximal promoter region.

Fig. 4

MCF-7 cells were transfected with lentiviral-based small hairpin RNA (shRNA). MCF-7 cells were transfected with NR2E3 shRNA or a non-specific control shRNA according to the manufacturer’s instructions. (A) Effect of NR2E3 shRNA on NR2E3 and ER protein expression. The NR2E3 and ERα protein levels were analyzed on immunoblots of cell lysates probed with anti-NR2E3 and anti-ERα antibodies. (B) NR2E3 loss significantly altered the transcriptional and epigenetic status of the ER proximal promoter region. Results are means ± SE for at least 3 replicated determinations, and significantly (P < .05) increased/attenuated (*) or decreased (**) responses are indicated.