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. Author manuscript; available in PMC: 2016 Sep 17.
Published in final edited form as: Toxicol Lett. 2015 Jul 3;237(3):228–236. doi: 10.1016/j.toxlet.2015.06.1708

Fig. 5. Inhibition of NR2E3 and ERα downregulation by antioxidant treatments and a model for the role of NR2E3 in BaP-mediated oxidative injury.

Fig. 5

(A) MCF-7 and T47D cells were pre-treated with GSH for 3 h, and BaP (5 μM) was then added for 48 h. The NR2E3 and ERα protein levels were analyzed on immunoblots of cell lysates probed with anti-NR2E3 and anti-ERα antibodies. (B) MCF-7 and T47D cells were pre-treated with NAC (10 mM) for 3 h, and BaP (5 μM) was then added for 48 h. The NR2E3 and ER protein levels were analyzed. (C) A model of the effects of BaP on the epigenetic status of the ER promoter. Results are means ± SE for at least 3 replicated determinations, and significantly (P < .05) increased/attenuated (*) or decreased (**) responses are indicated.