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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Cancer Lett. 2015 Jun 26;366(2):160–172. doi: 10.1016/j.canlet.2015.05.033

Figure 3. Specific knockdown of survivin expression induces cell cycle G2/M arrest in AML cells.

Figure 3

Kasumi-1 and HL-60 cells were infected with lentivirus containing either ConshRNA (Con) or SurshRNA (S3, S4, and S5). A & B, After 48 hr, cells were collected and subjected to flow cytometric analysis of cell cycle distribution. Data show the representative of three independent experiments. C, After 48 or 72 hr, cells were collected and subjected to Western blot analyses with specific antibody directed against survivin, γH2A.X (Ser139), H2A.X, P-CHK2 (Thr68), CHK2, or β-actin.