Figure 4.

Combinatorial genomic inversions and duplications by CRISPR with four sgRNAs. (A) Diagram of CRISPR with four sgRNAs targeted at the Pcdh α, β, and γ gene clusters. Shown are the inversions of the Pcdh α (B), β (C), γ (D), α/β (E), β/γ (F), and α/β/γ (G) gene clusters, as well as segmental duplications of the Pcdh α (H), β (I), γ (J), α/β (K), β/γ (L), and α/β/γ (M). The amplified upstream and downstream inversion junctions or segmental duplication junctions were sequenced. An example of Sanger sequencing chromatograms for each inversion or duplication is shown.