Table 1.
Transcriptome assembly and annotation statistics for F. arisanus
| Number of read pairs used in assembly (SRA accession number) | |
|---|---|
| Larvae (SRA: SRX689040) | 53 174 809 |
| Pupae (SRA: SRX689038) | 54 026 754 |
| Adult male (SRA: SRX689037) | 53 724 417 |
| Adult female (SRA: SRX689041) | 49 823 168 |
| Total | 210 749 148 |
| Normalized read pairs (in silico normalization) | 12 214 054 |
| Unfiltered assembly | |
| Number of unigenes | 57577 |
| N50 unigene length (longest transcript/unigene) (bp) | 2162 |
| Sum longest transcript/unigene (Mb) | 52.23 |
| Number of transcripts | 86118 |
| N50 transcript length (bp) | 3174 |
| Sum transcript length (Mb) | 117.14 |
| Transcripts per unigene | 1.50 |
| GC % | 40.45 |
| Filtered de novo assembly | |
| Number of unigenes | 8307 |
| N50 unigene length (longest transcript/unigene) (bp) | 4751 |
| Sum longest transcript/unigene (Mb) | 27.13 |
| Number of transcripts | 15346 |
| N50 transcript length (bp) | 4570 |
| Sum transcript length (Mb) | 50.62 |
| Isoforms per unigene | 1.85 |
| GC % | 41.37 |
| N50 protein length (amino acids) | 282 |
| Number of proteins with complete ORF (%) | 11115 (72.4) |
| Annotation statistics | |
| Number of proteins with Pfam domains identified | 11978 |
| Number of proteins with gene ontology terms | 9938 |
| Number of proteins with gene names | 14600 |