TABLE 2.

Estimates of Coexpression of GHSR mRNA in TH-Immunoreactive and CCK-Immunoreative Neurons¹

Nucleus	Approximate distance of section to interaural line2	% TH-IR neurons coexpressing GHSR	% CCK-IR neurons coexpressing GHSR
Arc (dorsal)3
	7.3 mm4	0.0 ± 0.0	—
	6.5 mm4	4.1 ± 1.8	—
	5.5 mm	15.2 ± 3.3	—
Arc (ventral)3
	7.3 mm	0.0 ± 0.0	—
	6.5 mm	65.3 ± 4.4	—
	5.5 mm	70.7 ± 2.8	—
EW
	4.3 mm	—	46.6 ± 4.6
	3.4 mm	—	39.8 ± 12.0
	2.8 mm	—	38.8 ± 21.5
VTA
	4.3 mm4	46.6 ± 4.6	35.5 ± 4.2
	3.4 mm4	23.2 ± 3.4	35.5 ± 11.7
	2.8 mm4	25.6 ± 3.9	42.7 ± 9.8
SNC
	4.3 mm	40.4 ± 4.3	53.2 ± 3.8
	3.4 mm	46.2 ± 6.9	43.8 ± 6.5
	2.8 mm	31.3 ± 9.4	38.3 ± 9.3
SNR
	4.3 mm	0.0 ± 0.0	—
	3.4 mm	17.3 ± 2.2	—
	2.8 mm	26.3 ± 3.4	21.9 ± 10.6
SNL
	4.3 mm	—	—
	3.4 mm	56.0 ± 9.0	53.9 ± 8.0
	2.8 mm5	36.1 ± 11.2	54.6 ± 15.5

¹The percentage of TH-IR and CCK-IR neurons coexpressing GHSR mRNA was determined, at three different levels through the tuberal hypothalamus and at three different levels through the midbrain. Strict criteria (described in Materals and Methods) were used to make these estimates. The data are reported as the mean percentage ± SEM for 3–5 different brains.

Dashes indicate regions without TH-IR or CCK-IR neurons.

²These approximate distances were determined by comparison to figures within the rat brain atlas of Paxinos and Watson (1998) and/or the study by Gonzalez-Hernandez and Rodriguez (2000).

³“Dorsal” and “ventral” refer to the dorsal and ventral subgroups of Dahlström-Fuxe’s A12 dopamine cell bodies located within the Arc; although these subgroups are difficult to distinguish at the 7.3 mm level, there nonetheless are no doubly labeled TH/GHSR neurons at this level.

⁴Representative low-power photomicrographs of these levels can be found in Figures 4 and 7, as follows: 7.3 mm, Fig. 4A; 6.5 mm, Fig. 4D; 4.3 mm, Fig. 7A; 3.4 mm, Fig. 7B; and 2.8 mm, Fig. 7C.

⁵Percentages listed at the 2.8 mm level of the SNL include TH-IR or CCK-IR neurons populating both the SNL and the retrorubral field.