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. Author manuscript; available in PMC: 2015 Aug 4.
Published in final edited form as: Sci Transl Med. 2015 Mar 18;7(279):279ra36. doi: 10.1126/scitranslmed.3010755

Fig. 6. Cisplatin-induced injury of PTE cells leads to colocalization of rhMG53 and annexin V at the plasma membrane.

Fig. 6

PTE cells were treated with cisplatin (50 μg/ml) for 3 hours. Rhodamine-labeled rhMG53 (0.1 mg/ml) or rhodamine-labeled BSA (0.1 mg/ml, as control) was then added to the cells. Immunostaining was performed with FITC–annexin V for labeling of exposed PS on the plasma membrane. Control PTE cells are negative for staining with rhMG53 or annexin V. PTE cells exposed to cisplatin show positive staining with rhMG53 and annexin V (bottom panels). Cells incubated with BSA show neither plasma membrane targeting nor intracellular localization of BSA, under control conditions or after cisplatin treatment. Scale bar, 10 μm.