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. 2015 Jul 29;8:1941–1948. doi: 10.2147/OTT.S82835

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© 2015 Lv et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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HIF-1α regulates MRP1 expression through a HRE in the MRP1 promoter.

Notes: Lovo cells were transfected with (A) the MRP1 promoter luciferase constructs (−998/+203, −481/+203, and −255/+203), (B) pLuc-MRP1w and HRE-mutated MRP1 promoter luciferase construct pLuc-MRP1m, and (C) pLuc-MRP1w and HIF-1α siRNA for 24 hours and then treated with or without 150 μmol/L CoCl₂ for 24 hours. Luciferase activity was analyzed by normalizing firefly luciferase activity with Renilla luciferase activity. *P<0.05.

Abbreviations: Ctr, control; HIF-1α, hypoxia-inducible factor-1α; HRE, hypoxia response element; LUC, luciferase; MRP1, multidrug resistance associated protein 1.