Figure 4.

HIF-1α binds to MRP1 promoter at HRE site.

Notes: (A) The sequence map of MRP1 promoter including HRE, primers for ChIP. (B) Lovo cells were treated with or without 150 μmol/L CoCl₂ and harvested at 24 hours for the ChIP assay as described in the “Methods” section. PCR primers encompassing HRE site were used.

Abbreviations: ChIP, chromatin immunoprecipitation; Fwd, forward; HIF-1α, hypoxia-inducible factor-1α; HRE, hypoxia response element; MRP, multidrug resistance associated protein; PCR, polymerase chain reaction; Rev, reverse; UTR, untranslated region; IgG, immunoglobulin gamma.