Table 1.
Primer name | Sequence (5′–3′) | Temperature (°C) | Product (bp) |
---|---|---|---|
qPCR primers | |||
GLUT1 Fwd | TGG GCA TGT GCT TCC AGT AT | 59 | 189 |
GLUT1 Rev | CCT CGG GTG TCT TGT CAC TT | ||
HIF-1α Fwd | GCC GCT GGA GAC ACA ATC AT | 55 | 181 |
HIF-1α Rev | GAA GTG GCT TTG GCG TTT CA | ||
MDR1 Fwd | GGC AAA GAA ATA AAG CGA CTG A | 58 | 417 |
MDR1 Rev | GGT GGA CAG GCG GTG AG | ||
MRP Fwd | AGC CAG AAA ATC CTC CAC GGT | 59 | 217 |
MRP Rev | CAT CGC CAT CAC AGC ATT GAC | ||
β-actin Fwd | CGG GAC CTG ACT GAC TAC CTC | 59 | 582 |
β-actin Rev | CTA GAA GCA TTT GCG GTG GA | ||
Cloning primers | |||
pLuc-MRPw Fwd | CTC AAG TAG CTG GGA CTA CAG GTG C | 60 | 1,221 |
pLuc-MRPw Rev | CAG AGC GGG TCG GAG CCA TC | ||
pLuc-MRP −481 Fwd | TCA GCG GAC CGG GTT CAA AT | 60 | 704 |
pLuc-MRP −255 Fwd | TGA AAA GTG GTC GCA GGG TGT GT | 60 | 478 |
pLuc-MRPm Fwd | CTGGGTTTCTTCCTTCATGAAATCAGAGACTTTACAGGATGAAATG | 58 | N/A |
ChIP primers | |||
MRP ChIP Fwd | CAG CCC TGC AAG TGA TTA GC | 57 | 225 |
MRP ChIP Rev | ACA CCC TGC GAC CA |
Abbreviations: ChIP, chromatin immunoprecipitation; Fwd, forward; GLUT1, glucose transporter 1 gene; HIF-1α, hypoxia-inducible factor-1α; MDR1, multidrug resistance 1 gene; MRP, multidrug resistance associated protein; qPCR, quantitative polymerase chain reaction; Rev, reverse; N/A, not available.