Table 1.

Primers list

Primer name	Sequence (5′–3′)	Temperature (°C)	Product (bp)
qPCR primers
GLUT1 Fwd	TGG GCA TGT GCT TCC AGT AT	59	189
GLUT1 Rev	CCT CGG GTG TCT TGT CAC TT
HIF-1α Fwd	GCC GCT GGA GAC ACA ATC AT	55	181
HIF-1α Rev	GAA GTG GCT TTG GCG TTT CA
MDR1 Fwd	GGC AAA GAA ATA AAG CGA CTG A	58	417
MDR1 Rev	GGT GGA CAG GCG GTG AG
MRP Fwd	AGC CAG AAA ATC CTC CAC GGT	59	217
MRP Rev	CAT CGC CAT CAC AGC ATT GAC
β-actin Fwd	CGG GAC CTG ACT GAC TAC CTC	59	582
β-actin Rev	CTA GAA GCA TTT GCG GTG GA
Cloning primers
pLuc-MRPw Fwd	CTC AAG TAG CTG GGA CTA CAG GTG C	60	1,221
pLuc-MRPw Rev	CAG AGC GGG TCG GAG CCA TC
pLuc-MRP −481 Fwd	TCA GCG GAC CGG GTT CAA AT	60	704
pLuc-MRP −255 Fwd	TGA AAA GTG GTC GCA GGG TGT GT	60	478
pLuc-MRPm Fwd	CTGGGTTTCTTCCTTCATGAAATCAGAGACTTTACAGGATGAAATG	58	N/A
ChIP primers
MRP ChIP Fwd	CAG CCC TGC AAG TGA TTA GC	57	225
MRP ChIP Rev	ACA CCC TGC GAC CA

Abbreviations: ChIP, chromatin immunoprecipitation; Fwd, forward; GLUT1, glucose transporter 1 gene; HIF-1α, hypoxia-inducible factor-1α; MDR1, multidrug resistance 1 gene; MRP, multidrug resistance associated protein; qPCR, quantitative polymerase chain reaction; Rev, reverse; N/A, not available.