Fig 3. The effects of finasteride (FIN) and thioacetamide (TAA) on expression of cytosolic (SOD1) and mitochondrial SOD izoenzyme (SOD2) in the (a) cortex and (b) the hippocampus.

For determination of expression polyclonal goat anti-SOD1 (1:500) and polyclonal goat anti-SOD2 (1:500) were used. After incubation with primary antibodies the membranes were incubated with the horseradish peroxidase (HRP) labeled secondary anti-goat antibody (1:2000) in TBST for 1 hour at room temperature. Five subsequent washes with 0.1% TBST were performed between each step. All membranes were stripped and re-probed with mouse monoclonal anti-actin antibodies (1:10000) to ensure that all wells were equally loaded. The signal was detected by enhanced chemiluminescence and subsequent exposure on an X-ray film. The significance of the difference was estimated by ANOVA with Fisher’s post hoc test (*p<0.05 and **p<0.01 vs. control, ##p<0.01 vs. TAA group, †p<0.01 vs. FIN group). For details see caption for Fig 1.