Yeast strains used were grown on YPO media. Data obtained with isolated peroxisomes (lane A), mitochondria (lane B) and endoplasmic reticulum (lane C) are shown. Line 1: Phospholipid pattern expressed as μg of individual phospholipids per mg protein. LP, lysophospholipids; PI, phosphatidylinositol; PS, phosphatidylserine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; CL, cardiolipin; PA, phosphatidic acid. Line 2: PC to PE ratio in different organelles and strains. Line 3: Fatty acid composition of different organelles and strains. Line 4: Ratio of saturated (SFA) to unsaturated (UFA) fatty acids in different organelles and strains. Line 5: Ergosterol (ERG) to phospholipid (PL) ratio in different organelles and strains. Line 6: Anisotropy values obtained with different organelles and strains. For experimental details see Materials and Methods section. For all experiments two independent biological samples were used which were analyzed 2 to 3 time, each.