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. 2015 Aug 4;10(8):e0133849. doi: 10.1371/journal.pone.0133849

Fig 8. Drought tolerance testing of SlDEAD31-overexpressing plants.

Fig 8

A water withholding assay was performed with 8-week-old plants for up to 21 d followed by a recovery period of 5 d. (A) Representative phenotypes of wild-type and SlDEAD31 overexpression plants at 0, 14 and 21 d after initiation of drought assay. (B) Survival rates of WT and transgenic plants after rewatering for 5 d following 21 d of drought assay. Each bar represents an average of eight plants ± SE. Asterisks indicate a significant difference from WT (p < 0.05). (C) and (D) Comparisons of chlorophyll content (C) and relative water content (RWC, D) of WT and transgenic plants at 0, 14 and 21 d after drought treatment. Values represent the means ± SE (n = 3). (E) Water loss rates of WT and transgenic plants. Similar leaves were excised and weighed at the indicated times. Water loss was calculated as the percentage of initial fresh weight. At least 15 leaves of similar developmental stages were excised and weighed at different times after the detachment. The data represent the means ± SE (n = 3).