Fig 4. ERK and Akt are involved in Tpl2-dependent IFNλ production in pDCs.

pDCs from WT and Tpl2 ^-/- mice were stimulated with R848 for 18 hr, and analyzed by intracellular staining for pAkt^Ser473. (A) Representative flow cytometry plots showing pAkt^Ser473 staining within pDCs. (B) Proportion of pAkt positive pDCs from 2 independent experiments. (C) pDCs were pretreated with inhibitors for 30 min before stimulation with CpG, and IFNλ levels were measured by ELISA. Data are representative of 2 (A-B) or 3 (C) independent experiments. Graphs show means±SD. * indicates p<0.05, ** indicates p<0.01.