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. 2015 Aug 4;10(8):e0134394. doi: 10.1371/journal.pone.0134394

Fig 5. Iba1 immunofluorescence of the ipsilateral dorsal horns from post-CCI day 7 young (YCCI) and middle-aged (MCCI) rats rendered in 3D.

Fig 5

Left Panels: optical sections (1.25 μm intervals through 25 μm thick sections) were acquired with a LSCM and 40x 1.4 NA objective lens and then recombined and rendered as three-dimensional images. Middle and Right Panels: image stacks (0.44 μm optical sections through 25 μm thick specimens) were obtained using a two-photon laser scanning confocal microscope and 100 X 1.4 NA objective lens. Each stack set was recombined to create the 3D rendering. A single plane of the 3D image is shown for each. Scale bar = 25 μm.