Fig 8. GFAP protein expression in young and middle-aged sham or CCI or naïve DRG.

(A) Immunoblots of DRG extracts were probed with antibody against GFAP or loading control GAPDH antibody. Upper panel: young and middle-aged naïve (YN and MN) DRG; lower panel: young and middle-aged sham (YS and MS) or CCI (YCCI and MCCI) DRG. (B) Typical immunofluorescence confocal images of 25 μm thick sections of DRG stained with GFAP antibody. Confocal images were acquired with a LSCM and 60 X 1.4 NA objective lens. L4 and L5 DRG were obtained ipsilateral to injury. Scale bar = 50 μm