Table 2. Gene Expression Profile of Young and Middle-Aged Sham and CCI LSC.

Gene	YS Mean	SD	n	YCCI Mean	SD	n	Fold YCCI/YS	p value
ATF3	1.60	0.05	4	8.79	1.68	5	5.49	0.0001
CCL2	1.30	0.30	9	4.53	1.04	9	3.49	0.0001
CD2	0.57	0.09	9	0.41	0.18	9	0.72	0.2834
CD3E	0.45	0.08	4	0.46	0.11	5	1.02	0.9991
CD45	3.65	0.63	4	7.18	1.68	5	1.97	0.0033
CD68	3.62	0.52	9	5.23	0.83	9	1.44	0.1862
IFN-γ	0.36	0.09	4	0.50	0.19	5	1.39	0.9441
IL10	1.31	0.26	4	1.38	0.69	5	1.05	0.9927
IL17a	0.38	0.12	4	0.25	0.13	5	0.66	0.9999
IL1β	0.23	0.05	9	0.31	0.13	9	1.35	0.2120
IL6	0.26	0.07	9	0.80	0.21	9	3.08	0.0001
TGFβ1*	5.94	0.52	4	8.03	2.03	5	1.35	0.0883
TNFα	0.38	0.11	4	0.45	0.05	5	1.18	0.6654
Gene	MS Mean	SD	n	MCCI Mean	SD	n	Fold MCCI/MS	p value
ATF3	2.28	0.34	5	5.98	0.44	5	2.62	0.0001
CCL2	1.93	0.37	9	3.42	1.00	10	1.77	0.0010
CD2	0.83	0.19	9	0.74	0.24	10	0.89	0.7400
CD3E	0.84	0.06	5	0.88	0.17	5	1.05	0.9470
CD45	7.05	1.05	5	8.31	1.22	5	1.18	0.3987
CD68	5.81	0.87	9	8.49	2.93	10	1.46	0.0068
IFN-γ	0.41	0.06	5	0.44	0.10	5	1.07	0.9747
IL10	1.41	0.28	5	1.42	0.17	5	1.01	0.9999
IL17a	0.30	0.07	5	0.26	0.05	5	0.87	0.9135
IL1β	0.30	0.06	9	0.26	0.09	10	0.87	0.8157
IL6	0.30	0.09	9	0.68	0.20	10	2.27	0.0001
TGFβ1	8.39	0.58	5	9.65	0.90	5	1.15	0.3811
TNFα	0.46	0.13	5	0.55	0.03	5	1.20	0.4756

LSC ipsilateral hemisections were removed on day 7. Expression levels in LSC samples were normalized to the geomean of Hprt1 and Pplb expression and the ratios multiplied by 100 and presented as the mean ratios +/- SD. Two-way ANOVA results are shown with contrasts and associated p values. Numbers of animals (n) assayed per group are shown. Bolded/italicized genes indicate specific contrast differences p < 0.05

* indicates difference trending to significance. YS = young sham, YCCI = young CCI, MS = middle-aged sham, MCCI = middle-aged CCI.