Table 7. Summary of multiplex assay results and immunofluorescence imaging of the LSC.

Comparison	Neuroinflammatory mRNA Markers	Iba1 Protein Levels	Density Microglia	P2/P1 Microglia
MN/YN	+ATF3, +CCL2, +CD2, +CD3E, +CD45, +CD68, +IL6, +TNFα, +TGFβ1	+++	~	+++
MS/YS	+CD2, +CD3E, +CD45, +CD68, +TGFβ1	+++	~	~
MCCI/YCCI	-ATF3,-CCL2, +CD2, +CD3E, +CD68	~	~	~
YCCI/YS	+++ATF3, +++CCL2, +CD45, +++IL6	+++	+++	+++
MCCI/MS	++ATF3, +CCL2, +CD68, +IL6	~	+++	~

LSC = lumbar spinal cord; MN = middle-aged naïve; YN = young naïve; MS = middle-aged sham; YS = young sham; MCCI = middle-aged CCI; YCCI = young CCI. + sign indicates ratios significantly > 1 but < 2-fold; ++ > 2-fold but < 3; +++ > 3-fold;–sign indicates ratios significantly < 1; and ~ sign indicates ratio ~ 1. Density of microglia refers to the dorsal horn, and for CCI, the ipsilateral side 7 days post-injury.