Table 8. Summary of multiplex assay results and immunofluorescence imaging of the DRG.

Comparison	Neuroinflammatory mRNA Markers	GFAP Protein Levels	GFAP + Satellite Glia
MN/YN	+ATF3	+++	MN only
MS/YS	CD45, TGFβ1 (both trending +)	+++	MS only
MCCI/YCCI	-CD68,-IL6	+++	MCCI only
YCCI/YS	+++ATF3, +CCL2, ++CD2, +CD45, ++CD68, ++GFAP, ++IFNG, +++IL17, ++IL1β, +++IL6, +++TNFα	~ low	~ ND
MCCI/MS	+CCL2, +++IL6	~ high	~ detected

LSC = lumbar spinal cord; MN = middle-aged naïve; YN = young naïve; MS = middle-aged sham; YS = young sham; MCCI = middle-aged CCI; YCCI = young CCI. + sign indicates ratio significantly > 1 but < 2-fold; ++ > 2-fold but < 3 fold; +++ > 3-fold;–sign indicates ratios significantly < 1; and ~ sign indicates ratio ~ 1. ND = not detected.