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. 2015 Aug 4;11(8):e1005332. doi: 10.1371/journal.pgen.1005332

Fig 5. Genic piRNA targeting is increased in the dysgenic germline.

Fig 5

(A) log10 Z-score heat map of genic (CDS) piRNA density for D. melanogaster orthologs (above a threshold of 5 piRNAs per CDS per 1 million mapped in at least one of four columns). Of these 105 genes, there is an excess of genic piRNAs in the dysgenic germline (89 genes with greatest genic targeting in dysgenesis, P<0.001) (B) Sense vs. Anti-sense abundance for piRNAs in genic piRNA class for one library (Sample 1). Some CDS regions are predominantly the source of anti-sense piRNAs, but the majority are biased as a source of sense strand piRNA (C) Distribution of expression levels (log 10 RPKM+0.01) for all genes in the genome and piRNA target genes (expression levels from non-dysgenic germline). Genic piRNA targets are derived from more highly expressed genes (p < 0.001). (D) Of 105 genes, the 89 that show excess genic piRNA in dysgenesis are also more lowly expressed in dysgenesis. Shown is the distribution of expression ratios (dysgenic:non-dysgenic) for all genes and genes that are increased as a source of genic piRNAs in dysgenesis (p < 0.001).