Table 4. Calculated time for a 4-log reduction of L. pneumophila sg. 1 env. associated with A.castellanii CCAP 1534/2 and Acanthamoeba sp. 155 after the exposure to different concentrations of free chlorine and temperatures.

Inactivation kinetics adjusted to first-order models. R² values showed the robustness of the models.

	Calculated time (min) to reduce 4 logs
Free chlorine	0.5 mg L -1	R2	1.2 mg L -1	R2	2.5 mg L -1	R2
L. pneumophila sg.1 env (Axenic)	5	0.96	—	—	—	—
L. pneumophila sg.1 env–A. castellanii CCAP 1534/2	490	0.85	152	0.76	43	0.79
L. pneumophila sg.1 env—Acanthamoeba sp. 155	38	0.54	17	0.64	23	0.82
Temperature	50°C	R2	55°C	R2	60°C	R2	70°C	R2
L. pneumophila sg.1 env (Axenic)	46	0.84	8	0.98	4	0.86	0.61	0.82
L. pneumophila sg.1 env–A. castellanii CCAP 1534/2	825	0.56	45	0.84	5	0.99	0.45	0.82
L. pneumophila sg.1 env—Acanthamoeba sp. 155	664	0.95	51	0.95	5	0.73	0.50	0.92