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. 2015 Aug 4;11(8):e1005370. doi: 10.1371/journal.pgen.1005370

Fig 5. Genes regulated by Dpp signalling independently of Salm/Salr.

Fig 5

(A) Control sal EPv -Gal4 UAS-GFP/+ wing. (B) Control third instar wing disc showing Spalt major expression (Red). (C-C’) sal EPv -Gal4 UAS-GFP/+; UAS-dad/+ wing disc showing Salm (red in C and C’) and GFP expression (green in C). (D) sal EPv -Gal4 UAS-GFP/+; UAS-dad/+ wing. (E) sal EPv -Gal4 UAS-GFP/+; UAS-dad/UAS-salm wing. (F) sal EPv -Gal4 UAS-GFP/+; UAS-dad/UAS-salr wing. Forced expression of Salm (E) or Salr (F) in the central region of sal EPv -Gal4 UAS-GFP;UAS-dad (D) discs partially rescues the size and pattern defects caused by Dad over-expression. (G-G’’’) CG32372 in situ hybridization in third instar discs of sal EPv -Gal4 UAS-GFP/+ (G), sal EPv -Gal4 UAS-GFP/UAS-salm-i; UAS-salr-i/+ (G’), sal EPv -Gal4 UAS-GFP/+; UAS-dad/+ (G’’) and sal EPv -Gal4 UAS-GFP/+; UAS-dad/UAS-salm (G’’’). (H-H’’’) CG17278 in situ hybridization in third instar discs of sal EPv -Gal4 UAS-GFP/+ (H), sal EPv -Gal4 UAS-GFP/UAS-salm-i; UAS-salr-i/+ (H’), sal EPv -Gal4 UAS-GFP/+; UAS-dad/+ (H’’) and sal EPv -Gal4 UAS-GFP/+; UAS-dad/UAS-salm (H’’’). The over-expression of Salm does not rescue the loss of CG32372 or CG17278 expression caused by increased Dad expression.