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. 2015 Aug 4;11(8):e1005370. doi: 10.1371/journal.pgen.1005370

Fig 7. Phenotypic analysis of genes with reduced expression in sal knockdown discs.

Fig 7

(A) Fraction of genes showing a wing mutant phenotype (Y; green) and no phenotype (N; red) from a total of 139 UAS-RNA-i/Gal4 combinations. Genes not analysed of the STG- class are shown in blue (—). (B) Frequency of mutant wings showing defects in both wing size and vein pattern (blue; S-P), changes in wing size (S; red), defects in vein formation with minor or no effect in wing size (V; green) and other morphological defects in the wing (O; purple) from a total of 64 UAS-RNA-i/Gal4 combinations. (C) Wild type wing. (D) sal EPv -Gal4/UAS-salm-i; UAS-salr-i/+ wing. (E-P) Representative examples of adult wings knockdown for genes showing reduced expression by in situ hybridization in salm-i/salr-i wing discs. UAS-dicer2/+; sal EPv -Gal4/UAS-CG12182-i grown at 29°C (E); sal EPv -Gal4 UAS-GFP/UAS-CG4322-i (F), UAS-dicer2/+; sal EPv -Gal4 / UAS-CG8675-i (G), UAS-dicer2/+; sal EPv -Gal4/+; UAS-CG11607-i/+ (G), dicer2/+; sal EPv -Gal4/ UAS-CG16756-i (I), UAS-dicer2/+; nub-Gal4/UAS-CG4570-i (J), UAS-dicer2/+; nub-Gal4/UAS-CG31436-i (K), UAS-dicer2/+; nub-Gal4/+; UAS-CG9333-i/+ (L), UAS-dicer2/+; nub-Gal4/UAS-CG8768-i (M), UAS-dicer2/+; sal EPv -Gal4/UAS-CG30069-i (N), UAS-dicer2/+; sal EPv -Gal4/+; UAS-CG8084-i/+ (O) and UAS-dicer2/+; sal EPv -Gal4/ UAS-CG1869-i at 29°C (P).