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. 2015 Aug 4;11(8):e1005078. doi: 10.1371/journal.ppat.1005078

Fig 5. IgA protease of ST-11 isolates promotes sustained activation of JNK and apoptosis of epithelial cells.

Fig 5

(A) Hec-1B cells were infected with the indicated strains. At each time point, samples were lysed and immunoblotted with rabbit polyclonal anti-phospho-JNK antibodies (upper panel). The membranes were subsequently stripped and re-probed with rabbit polyclonal anti-JNK antibodies as protein loading controls. Blots are representative of three separate experiments with similar results. (B) Cells were left uninfected or infected with the indicated strains for 9 h then analysed for apoptosis using annexin V staining and flow cytometry. Histogram bars represent the mean ± SD from three independent experiments. **, P < 0.01, ***, P <0.001.