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. 2015 Aug 4;11(8):e1005073. doi: 10.1371/journal.ppat.1005073

Fig 5. ROCK-I interacts with PDK1 and phosphorylates PDK1.

Fig 5

(A) Immunoprecipitation of ROCK-I and immunoblotting of PDK1 in uninfected 1C115-HT or Fk-1C115-HT treated or not with Y-27632 (100 μM, 1h) as well as in 1C115-HT transfected with S241A PDK1 mutant. (B) Cell 32P metabolic labeling followed by PDK1 immunoprecipitation and western blotting for PDK1 phosphorylation level in uninfected 1C115-HT or Fk-1C115-HT treated or not with Y-27632 (100 μM, 1h) as well as in 1C115-HT transfected with S241A PDK1 mutant. Values are the mean ± s.e.m. # P < 0.05 versus non treated uninfected cells. ## P < 0.05 versus non treated infected cells.