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. Author manuscript; available in PMC: 2015 Aug 4.
Published in final edited form as: Biochem Pharmacol. 2015 Jan 29;94(2):69–78. doi: 10.1016/j.bcp.2015.01.009

Figure 1. CUR inhibited anchorage-independent growth of HT29 cells.

Figure 1

(A) HT29 cells (8,000 cells/well) were plated in soft agar containing 0.1% DMSO (Control) and CUR (2.5 μM or 5 μM) in 6-well plates for 14 days. The colonies were counted under a microscope and analyzed using ImageJ software. The colony number percentage was calculated by dividing the number of colonies formed in the CUR treatment groups by the number of colonies formed in the control group. Representative images of each group under a microscope are shown in the left panel. Graphical data are presented as the mean ± SEM of triplicate results from three independent experiments. * P<0.05 versus the control group and ** P<0.01 versus the control group. (B) HT29 cells were firstly treated with 0.1% DMSO (Control) and CUR (2.5 μM or 5 μM) for 5 days. On day 5, pretreated cells (8,000 cells/well) were transferred and grown in agar for additional 14 days without presence of CUR. The colonies were counted under a microscope and analyzed using ImageJ software. The colony number percentage was calculated by dividing the number of colonies formed with pretreated cells by the number of colonies formed in the control group. Representative images of each group under a microscope are shown in the left panel. Graphical data are presented as the mean ± SEM of triplicate results from three independent experiments. * P<0.05 versus the control group and ** P<0.01 versus the control group. (C) HT29 Cells were plated in 96-well plates at an initial density of 1,000 cells/ well for 24 h. The cells were then incubated in fresh medium with the presence of CUR (1-25 μM) for 5 days. Cell viability was determined by MTS assay. The data are presented as mean ± SEM of three independent experiments. * P<0.05 versus control group, ** P<0.01 versus control group.