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. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: Cell Rep. 2015 Feb 5;10(5):740–754. doi: 10.1016/j.celrep.2015.01.007

Figure 6. Yap and β-catenin are direct substrates of PKCζ.

Figure 6

(A and B) Interaction of PKCζ with Yap (A) and β-catenin (B) in co-transfection experiments in HEK293T cells. (C) In vitro interaction of recombinant PKCζ with recombinant Yap and β-catenin. (D) Endogenous interaction of PKCζ and components of Axin1 complex in HEK293T cells. (E) Endogenous association of Axin1 with PKCζ and GSK3β in Wnt3a treated-HEK293T cells. (F and G) In vitro phosphorylation of Yap (F) and β-catenin (G) by PKCζ with [γ-32P]-ATP. (H) Western blot of thiophosphate ester after YAP phosphorylation by PKCζ with ATPγS. (I) Western blot of S45-β-catenin phosphorylated by PKCζ in vitro. (J and K) Western blot of β-catenin phosphorylation in IECs from Lgr5-PKCζWT/WT and Lgr5-PKCζ−/− mice (J) and in crypt organoids from WT and PKCζ KO mice (K). Results are representative of three experiments. See also Figure S3.