Table 2.
Effects of types and concentrations of auxins along with optimal concentration of cytokinin on multiples shoot production in H. enneaspermus after 8 weeks of culture
| Auxins concentration (mg/l) | Multiple shoot regeneration frequency (%) | No. of multiple shoots/explant | Length of the shoot (cm) | |||
|---|---|---|---|---|---|---|
| Leaf | Node | Leaf | Node | Leaf | Node | |
| Control | 82.7a | 78.7b | 28.24 ± 0.49d | 18.25 ± 0.33f | 5.41 ± 0.22a | 4.85 ± 0.03b |
| IBA | ||||||
| 0.5 | 79.4b | 75.3b | 26 ± 0.31ef | 21 ± 0.83e | 5.30 ± 0.17a | 5.10 ± 0.28ab |
| 1.0 | 83.3a | 79.4b | 30.2 ± 1.11c | 25.4 ± 0.50c | 5.40 ± 0.11a | 5.20 ± 0.11ab |
| 1.5 | 77.7b | 72.2b | 24.4 ± 0.50fg | 18.6 ± 0.50f | 5.36 ± 0.12a | 5.14 ± 0.12ab |
| 2.0 | 73.8b | 68.1c | 20.8 ± 0.58h | 15.4 ± 0.50g | 5.20 ± 0.08a | 5.26 ± 0.10ab |
| NAA | ||||||
| 0.5 | 80.1a | 75.3b | 26.4 ± 0.50e | 21.4 ± 0.97e | 5.44 ± 0.10a | 5.22 ± 0.11ab |
| 1.0 | 84.4a | 79.7b | 30.2 ± 0.66c | 25.4 ± 0.50c | 5.50 ± 0.05a | 5.38 ± 0.08a |
| 1.5 | 88.6a | 83.5a | 34.2 ± 0.58a | 29.4 ± 0.50a | 5.66 ± 0.16a | 5.40 ± 0.15a |
| 2.0 | 82.7a | 76.6b | 28.4 ± 0.50cd | 23.6 ± 0.50d | 5.52 ± 0.17a | 5.30 ± 0.16ab |
| IAA | ||||||
| 0.5 | 76.5b | 72.7b | 24.2 ± 0.48g | 19.2 ± 0.37f | 5.36 ± 0.15a | 5.14 ± 0.12ab |
| 1.0 | 80.8a | 76.8b | 28.4 ± 0.67cd | 23.2 ± 0.37d | 5.44 ± 0.08a | 5.22 ± 0.19ab |
| 1.5 | 85.2a | 80.5a | 32 ± 0.31b | 27.2 ± 0.37b | 5.50 ± 0.15a | 5.32 ± 0.16ab |
| 2.0 | 79.1b | 73.4b | 26.2 ± 0.48e | 21.4 ± 0.50e | 5.28 ± 0.5a | 5.20 ± 0.15ab |
Control: MS medium supplemented with 4 mg/l BA or 3 mg/l BA for leaf and nodal explants respectively. Values represent the mean ± standard error of three experiments. Mean values followed by the same letters within a column are not significantly different according to Duncan’s multiple range test at 5 % level